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Sequencing

The Saccharomyces cerevisiae genome is approximately 12 Mb, organized in 16 chromosomes.

The University of Iowa determined the complete nucleotide sequence of the two-micron circular plasmid from S. cerevisiae strain A364A^[1].

Stanford University sequenced the genome of S. cerevisiae strain YJM789 at approximately 10X coverage using whole genome shotgun (WGS) sequencing^[2].

The Australian Wine Research Institute sequenced the S. cerevisiae strain AWRI1631 genome using whole genome shotgun (WGS) sequencing. They generated 2484 contigs with annotation^[3].

The Washington University School of Medicine (WashU) sequenced the S. cerevisiae strain M22 genome at approximately 2.6X coverage and strain YPS163 genome at approximately 2.8X coverage using whole genome shotgun (WGS) sequencing^[4].

The Broad Institute sequenced the genome of the natural isolate S. cerevisiae strain RM11-1a at approximately 10X coverage using whole genome shotgun (WGS) sequencing. They generated a S. cerevisiae strain RM11-1a genome assembly, which will undergo automated annotation.

An international consortium completed the sequencing of the S. cerevisiae strain S288C genome in 1996. It was the first eukaryotic genome to be completely sequenced.

The Stowers Institute For Medical Research is sequencing the S. cerevisiae strain S288C genome using Solexa sequencing technology.

Annotation

Updates to the Saccharomyces cerevisiae sequence and annotation are collected and maintained in the Saccharomyces Genome Database (SGD).

References

1. ↑ Hartley JL & Donelson JE (1980) Nucleotide sequence of the yeast plasmid. Nature 286: 860-5 PubMed
2. ↑ Wei W et al. (2007) Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789. Proc Natl Acad Sci U S A 104: 12825-30 PubMed
3. ↑ Borneman AR et al. (2008) Comparative genome analysis of a Saccharomyces cerevisiae wine strain. FEMS Yeast Res 8: 1185-95 PubMed
4. ↑ Doniger SW et al. (2008) A catalog of neutral and deleterious polymorphism in yeast. PLoS Genet 4: e1000183 PubMed