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Candida albicans
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Sequencing
The Stanford University Genome Technology Center sequenced and annotated the complete mitochondrial genome of Candida albicans[1].
The Sanger Center carried out a pilot sequencing project on the Candida albicans strain 1161 genome. They sequenced ten cosmids for this project.
The Broad Institute sequenced the genome of Candida albicans strain WO-1 at 10X coverage using whole genome shotgun (WGS) sequencing.
Stanford University sequenced the heterozygous diploid genome of Candida albicans strain SC5314 using whole genome shotgun (WGS) sequencing. They initially assembled the sequence using PHRAP, which resulted in an assembly (Assembly 6) in which the sum of the contigs significantly exceeded the haploid genome size due to the heterozygosity. They then developed computational methods to reconstruct the diploid genome sequence from the PHRAP assembly, which resulted in an assembly (Assembly 19) that was in good agreement with available physical mapping data[2].
Annotation
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References
- ↑ Anderson JB et al. (2001) Infrequent genetic exchange and recombination in the mitochondrial genome of Candida albicans. J Bacteriol 183: 865-72 PubMed
- ↑ Jones T et al. (2004) The diploid genome sequence of Candida albicans. Proc Natl Acad Sci U S A 101: 7329-34 PubMed
